CRISPR has revolutionized the generation of transgenic animals. It has significantly reduced the time and the cost required to generate genetically engineered mice.
The requesting PI is responsible for obtaining IACUC approval and mouse space before any work to generate the new mouse line commences.
Guarantees: The core will surgically transfer at least 120 embryos or produce 2 founders for each project, whichever comes first. We will repeat the procedure for half the price if unsuccessful at first attempt. We are unable to guarantee a positive outcome. We cannot guarantee a founder mouse when the mutation is embryonic lethal. We cannot guarantee that the mutation will have the desired phenotype.